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高远生 博士
研究室负责人 Yuansheng Gao, Ph.D.
Phone：010-82805038
E-mail: ygao@bjmu.edu.cn
教育经历
Education
1982 北京医学院医学硕士 (生理学)
Beijing Medical University, Master of Medicine (Physiology)
1990 美国Mayo研究生院博士 (生理学)
Mayo Graduate School, USA, PhD (Physiology)
工作经历
Professional Experience
2002-present: 北京大学医学部生理学与病理生理学系教授
Professor, Department of Physiology and Pathophysiology
1999-2002: 美国加州大学洛杉矶分校医学院海港医学中心研究副教授
Adjunct Associate Professor, Harbor-UCLA Medical Center, CA, USA
1992-1999: 美国加州大学洛杉矶分校医学院海港医学中心研究副教授
Assistant Adjunct Professor, Harbor-UCLA Medical Center, CA, USA
1991-1992: 美国德克萨斯州贝勒医学院研究讲师
Research Instructor, Baylor College of medicine, Houston, TX, USA.
研究室成员：
窦豆 讲师，北京大学医学部病理生理学博士
何琼 讲师，香港中文大学医学院生理学博士
刘利梅 讲师，哈尔滨医科大学病理生理学硕士
余晓星 技师,首都师范大学细胞生物学硕士
郑晓旭 博士生 01级，北京大学医学部基础医学学士
应磊 博士生 08级，浙江大学医学院生理学硕士
刘娟 硕博连读生 09级，山东泰安医学院医学学士
研究室概述：
循环糸统对组织血流的调节主要通过血管的收缩与舒张实现，我们室的主要研究邻域为生理与病生理条件下血管反应的调节机制，特别是一氧化氮-鸟苷酸环化酶-环鸟苷酸蛋白激酶，RhoA与RhoA蛋白激酶，肌球蛋白磷酸酶，活性氧，与活性氮等ROCK对血管的收缩与舒张的调节机制。我们近年来的工作表明环鸟苷酸蛋白激酶在硝酸甘油与一氧化氮引起的冠状动脉舒张起关键作用，环鸟苷酸蛋白激酶的下调是硝酸甘油耐受产生的机制之一。我们的工作还表明肌球蛋白磷酸酶亮氨酸拉链表达阳性亚单位的蛋白酶体降解增加可导致冠状动脉与肺动脉对一氧化氮舒张减弱。
Research Description:
Vasoconstriction and vasodilatation are the major means by which the blood flow to the tissues is regulated. Our research interest is primarily in the mechanisms underlying the vasoreactivity under physiological and pathophysiological conditions, in particular, the role of nitric oxide (NO), soluble guanylyl cyclase (sGC), cGMP-dependent protein kinase (PKG), RhoA/Rho kinase, myosin light chain phosphatase (MLCP), and reactive oxygen/nitrogen species. Our recent studies demonstrate that PKG plays a major role in relaxation of coronary arteries and veins induced by nitroglycerin and NO. The downregulation of PKG expression and activity contributes to the development of nitrate tolerance. Our studies also show that an enhanced degradation by proteasomes of the leucine-zipper positive isoform of the regulatory subunit of MLCP may lead to diminished response of coronary and pulmonary arteries to nitric oxide.
Selected Publications (2000-Present):
1. Gao Y, Dhanakoti S, Trevino EM, Sander FC, Portuga AM, Raj JU. Effect of oxygen on cyclic GMP-dependent protein kinase-mediated relaxation in ovine fetal pulmonary arteries and veins. Am J Physiol, 285: L611-L618, 2003.
2. Gao Y, Dhanakoti S, Trevino EM, Wang X, Sander FC, Portuga AD, Raj JU. Role of cGMP-dependent protein kinase in development of tolerance to nitric oxide in pulmonary veins of newborn lambs. Am J Physiol 286: L786–L792, 2004.
3. Gao Y, Raj JU. Parathyroid hormone-related protein effects on the responsiveness of newborn ovine pulmonary arteries and veins. Am J Physiol 289: L60–L66, 2005.
4. Gao Y, Raj JU. Role of veins in regulation of the pulmonary circulation. Am J Physiol 288: L213-L226, 2005.
5. Wang X, Tong M, Chinta S, Raj JU, Gao Y. Hypoxia-induced reactive oxygen species downregulate ETB-receptor mediated contraction of rat pulmonary arteries. Am J Physiol 290: L570–L578, 2006.
6. Gao Y and Raj JU. cGMP-dependent protein kinase in regulation of the pulmonary circulation. Current Respiratory Medcine Reviews, 2: 373-381, 2006.
7. Gao Y, Portugal AD, Negash S, Zhou W, Longo LD, and Raj JU. Role of Rho kinases in PKG-mediated relaxation of pulmonary arteries of fetal lambs exposed to chronic high altitude hypoxia. Am J Physiol, 272: L678-L684, 2007.
8. Qin X, Zheng X, Qi H, Dou D, Raj JU, and Gao Y. cGMP-dependent protein kinase in regulation of basal tone and in nitroglycerin and nitric oxide induced relaxation in porcine coronary artery. Pflügers Archiv - Eur J Physiol 454: 913-923, 2007.
9. GuanY, Zhang Y, Wu J, Qi Z, YangG, Dou D, Gao Y, Chen L, Zhang X, Davis LS, Wei M, Fan X, Carmosino M, Hao C, Imig JD, Breyer RM and Breyer MD. Anti-hypertensive effect of selective prostaglandin E2 EP1 receptor targeting. J Clin Invest 117: 2496-505, 2007.
10. Qi H, Zheng X, QinX, Dou D, Xu H, Raj JU, Gao Y. PKG regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Br J Pharmacol, 152: 1060-1069, 2007.
11. Dou D, ZhengX, Qin X, QiH, Liu L, Raj JU, GaoY. Role of PKG in development of nitroglycerin tolerance of porcine coronary arteries. Br J Pharmacol, 153: 497-507, 2008.
12. GaoY, Portugal AD, LiuJ, Negash S, Zhou W, Tian J, Xiang R, Longo LD and Raj JU. Preservation of cGMP-induced relaxation of pulmonary veins of fetal lambs exposed to chronic high altitude hypoxia: Role of PKG and Rho kinase. Am J Physiol 295: L889-L896, 2008.
13. Gao Y, Raj JU. Endothelium and Perinatal Pulmonary Circulation. Chapter 23, in “The Pulmonary Endothelium – Function in Health and Disease”. eds. Voelkel NF, Rounds S. John Wiley & Sons, in press. |
