血管分子生物学与生物工程学研究室

　　兼任亚洲血管生物学会秘书长、中国生理学会学术工作委员会副主任委员、中国病理生理学会受体专业委员会副主任委员、中国生物学会及分子生物学会脂蛋白专业委员会委员；国家自然科学基金委医学部专家咨询委委员。Faculty1000委员、PPAR Research副主编、Nuclear Receptors（亚太区）主编、Circulation Journal副主编、Cardiovasc Drug & Therapy、J Diabetes、世界临床医学(Lancet中文版)等杂志编委。

　　主要在研科研基金 (Research fundings)：

　　国家自然科学基金 (30670848)：Kr？ppel-样因子4在血管内皮机械生物信号传导中的作用-课题负责人

　　科技部重大基础研究973项目课题 (2006503906):血管病变与二型糖尿病发病机制-课题负责人

　　国家自然科学基金委重大项目(30890040)：代谢性核受体与糖脂代谢-项目负责人

　　国家自然科学基金委创新群体项目 (30821001)：代谢综合症及其血管病变-课题负责人

　　北京大学医学部-工学院联合研究基金：以血管新生为靶点的小分子调节microRNA的研究-课题负责人

　　科技部重大基础研究973项目课题：细胞膜重要脂质代谢产物对重大疾病病理生理过程的调控 –课题骨干

　　研究室成员 staff

　　李彦辉助理研究员

　　刘雅涵助理研究员

　　赵蓓蕾中级技师

　　研究室概述 Research description

　　本研究室采用分子生物学、功能基因组学方法通过培养细胞和整体动物模型探讨代谢相关的血管疾病的发病机制，以期寻找其治疗的新靶点。主要研究方向包括：

　　1）动脉粥样硬化及血管再狭窄分子病理机制和治疗靶点：主要研究生长因子、脂肪因子对血管内皮细胞、平滑肌细胞增殖与迁移及血管损伤后新生内膜形成的影响；

　　2）血管机械-生物信号传导机制：主要集中研究血流机械剪应力调节血管内皮细胞基因表达的信号通路和转录机制；

　　3）代谢性核受体对糖脂代谢的调控机制：主要研究PPARa、d、g糖脂代谢相关靶基因的调控机制及其在脂肪合成和血管内皮细胞功能调节中的作用

　　研究论文 (Selected Publication)

　　1. Zhang, Y., et al., Inhibition of bone morphogenic protein 4 restores endothelial function in db/db diabetic mice. Arterioscler Thromb Vasc Biol, 2014. 34(1): p. 152-9.

　　2. Xiao, L., Y. Liu, and N. Wang, New paradigms in inflammatory signaling in vascular endothelial cells. Am J Physiol Heart Circ Physiol, 2014. 306(3): p. H317-25.

　　3. Wang, L., et al., Allergic asthma accelerates atherosclerosis dependent on Th2 and Th17 in apolipoprotein E deficient mice. J Mol Cell Cardiol, 2014. 72: p. 20-7.

　　4. Liu, Y., et al., Rosiglitazone Attenuated Endothelin-1-Induced Vasoconstriction of Pulmonary Arteries in the Rat Model of Pulmonary Arterial Hypertension via Differential Regulation of ET-1 Receptors. PPAR Res, 2014. 2014: p. 374075.

　　5. Huang, Y., et al., Peroxisome Proliferator-Activated Receptor gamma Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells. PPAR Res, 2014. 2014: p. 740121.

　　6. Cheang, W.S., et al., Metformin protects endothelial function in diet-induced obese mice by inhibition of endoplasmic reticulum stress through 5' adenosine monophosphate-activated protein kinase-peroxisome proliferator-activated receptor delta pathway. Arterioscler Thromb Vasc Biol, 2014. 34(4): p. 830-6.

　　7. Zhang, Y., et al., Kruppel-like factor 4 transcriptionally regulates TGF-beta1 and contributes to cardiac myofibroblast differentiation. PLoS One, 2013. 8(4): p. e63424.

　　8. Xie, P., et al., Ezetimibe inhibits hepatic Niemann-Pick C1-Like 1 to facilitate macrophage reverse cholesterol transport in mice. Arterioscler Thromb Vasc Biol, 2013. 33(5): p. 920-5.

　　9. Wang, Y., et al., The g0/g1 switch gene 2 is an important regulator of hepatic triglyceride metabolism. PLoS One, 2013. 8(8): p. e72315.

　　10. Wang, X., et al., C-reactive protein reduces protein S-nitrosylation in endothelial cells. Mol Cell Biochem, 2013. 375(1-2): p. 131-8.

　　11. Wang, X., et al., Shear stress activation of nuclear receptor PXR in endothelial detoxification. Proc Natl Acad Sci U S A, 2013. 110(32): p. 13174-9.

　　12. Liu, Y., et al., Epigallocatechin-3-O-gallate, a green tea polyphenol, induces expression of pim-1 kinase via PPARgamma in human vascular endothelial cells. Cardiovasc Toxicol, 2013. 13(4): p. 391-

　　13. Liu, Y., et al., Metabonomic profiling revealed an alteration in purine nucleotide metabolism associated with cardiac hypertrophy in rats treated with thiazolidinediones. J Proteome Res, 2013. 12(12): p. 5634-41.

　　14. Liu, Y., et al., Response to overexpression of 5-hydroxytryptamine 2B receptor gene in pulmonary hypertension: still a long way to understand its transcriptional regulation. Hypertension, 2013. 61(4): p. e30.

　　15. Wang, Y., et al., Kruppel-like factor 4 is induced by rapamycin and mediates the anti-proliferative effect of rapamycin in rat carotid arteries after balloon injury. Br J Pharmacol, 2012. 165(7): p. 2378-88.

　　16. Tian, X.Y., et al., PPARdelta activation protects endothelial function in diabetic mice. Diabetes, 2012. 61(12): p. 3285-93.

　　17. Liu, Y., et al., Peroxisome proliferator-activated receptor-gamma ameliorates pulmonary arterial hypertension by inhibiting 5-hydroxytryptamine 2B receptor. Hypertension, 2012. 60(6): p. 1471-8.

　　18. Li, X., et al., LIF maintains progenitor phenotype of endothelial progenitor cells via Kruppel-like factor 4. Microvasc Res, 2012. 84(3): p. 270-7.

　　19. Chen, L., et al., Inactivation of the E-prostanoid 3 receptor attenuates the angiotensin II pressor response via decreasing arterial contractility. Arterioscler Thromb Vasc Biol, 2012. 32(12): p. 3024-32.

　　20. Chen, L., et al., Oral Administration of Shark Type II Collagen Suppresses Complete Freund's Adjuvant-Induced Rheumatoid Arthritis in Rats. Pharmaceuticals (Basel), 2012. 5(4): p. 339-52.

　　21. Zhu, S., et al., Targeted disruption of the prostaglandin E2 E-prostanoid 2 receptor exacerbates vascular neointimal formation in mice. Arterioscler Thromb Vasc Biol, 2011. 31(8): p. 1739-47.

　　22. Wang, Y., et al., Kruppel-like Factor 4 is Induced by Rapamycin and Mediates the Anti-proliferative Effect of Rapamycin in Balloon-injured Rat Carotid Arteries. Br J Pharmacol, 2011.

　　23. Wang, N.P., et al., Role of Peroxisome Proliferator-Activated Receptor-gamma in Atherosclerosis - An Update. Circulation Journal, 2011. 75(3): p. 528-535.

　　24. Li, J., et al., Pancreatic-derived factor promotes lipogenesis in the mouse liver: role of the Forkhead box 1 signaling pathway. Hepatology, 2011. 53(6): p. 1906-16.

　　25. Xu, G., et al., Regulation of gastric hormones by systemic rapamycin. Peptides, 2010. 31(12): p. 2185-92.

　　26. Wang, K.C., et al., Role of microRNA-23b in flow-regulation of Rb phosphorylation and endothelial cell growth. Proceedings of the National Academy of Sciences of the United States of America, 2010. 107(7): p. 3234-3239.

　　27. Tian, J., et al., Rosiglitazone attenuates endothelin-1-induced vasoconstriction by upregulating endothelial expression of endothelin B receptor. Hypertension, 2010. 56(1): p. 129-35.

　　28. Qin, X., et al., MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells. Proc Natl Acad Sci U S A, 2010. 107(7): p. 3240-4.

　　29. Wang, N., Verna, L., Hardy, S., Forsayeth, J., Zhu, Y., Ma, K.S., Stemerman, M.B. Adenovirus-mediated Overexpression of c-Jun and c-Fos Induces Intercellular Adhesion Molecule-1 and Monocyte Chemo-attractant Protein-1 in Human Endothelial Cells. Arterioscler Thromb Vasc Biol. 1999; 19:2078-2084

　　30. Liu,Y., Zhu, Y., Rannou, F., Formentin, K., Zeng, L-F., Yuan X, BM. Forman, Wang N., Chien, S., Shyy J. Y-J. Shear Stress Increases the Endothelial Release of Ligands for Peroxisome Proliferator-Activated Receptor-gamma. Circulation 2004 110:1128-33

　　31. Zhu, Y.,Liao, H., Xie, XF., Yuan, Y., Lee, T-S., Wang, N., Wang, X., Shyy, J.Y-J., Stemerman, MB.Oxidized LDL downregulates ATP-binding cassette transporter-1 in human vascular endothelial cells via inhibiting LXR. Cardiovas Res 2005；68:425-432.

　　32. Wang,N.; Miao,H.; Li,Y.S.; Zhang,P.; Haga,J.H.; Hu,Y.; Young,A.; Yuan,S.; Nguyen,P.; Wu,C.C.; Chien,S. Shear stress regulation of Kruppel-like factor 2 expression is flow pattern-specific. Biochem Biophys Res Commun. 2006; 341:1244-1251

　　33. Li J, Wang N.Peroxisome proliferator-activated receptor-gamma in vascular biology. Cardiovasc Hematol Disord Drug Targets. 2007;7:109-17.

　　34. Gao DF, Niu XL, Hao GH, Peng N, Wei J, Ning N, Wang N. Rosiglitazone inhibits angiotensin II-induced CTGF expression in vascular smooth muscle cells-Role of PPAR-gamma in vascular fibrosis. Biochem Pharmacol. 2007, 73:185-97

　　35. Qin X, Tian J, Zhang P, Fan Y, Chen L, Guan Y, Fu Y, Zhu Y, Chien S, Wang N. Laminar shear stress up-regulates the expression of stearoyl-CoA desaturase-1 in vascular endothelial cells. Cardiovasc Res. 2007; 74:506-14.

　　36. Dwarakanath RS, Sahar S, Lanting L, Wang N, Stemerman MB, Batarajan R, Reddy MA. Viral Vector Mediated 12/15-Lipoxygenase Overexpression in Vascular Smooth Muscle Cells Enhances Inflammatory Gene Expression and Migration. J Vasc Res. 2007; 45:132-142

　　37. Ai D, Fu Y, Guo DL, Tanaka H, Wang N, Tang CS, Hammock BD, Shyy JY-J, Zhu Y. Angiotensin II Upregulates Soluble Epoxide Hydrolase in Vascular Endothelium In vitro and In vivo. Proc Natl Acad Sci USA. 2007; 104:9018-9023

　　38. Zhu M, Fu Y, Wang N, Guan Y, Tang CS, Shyy J.Y-J., Zhu Y. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells In vitro and In vivo. Arterio Thromb Vasc Biol. 2008;28:527-33

　　39. Fan Y, Wang Y, Zhang H, Qin X, Zhu Y, Guan Y, Wang X, Staels B, Chien S, Wang N.Suppression of Pro-inflammatory Adhesion Molecules by PPAR-d in Human Vascular Endothelial Cells. Arterioscler Thromb Vasc Biol. 2008;28:315-2

　　40. Hao, G, Niu, X, Gao, D, Wei J, Wang N. PPAR-g ligands suppress angiotensin II-induced plasminogen activator inhibitor-1 and extracellular matrix production in rat cardiac fibroblasts. British J Pharmacol, 2008;153:1409-1419.

打印本页关闭窗口